AKIPRESS.COM - The fortified Toyota Land Cruiser slipped and bounced in the muddy hollows of the rain-drenched Mongolian steppe. The driver, a native Mongolian man named Inka who spoke little English, slowly engineered the vehicle along what just two days earlier was a dusty pair of dirt tracks, says DukeToday.
In the back seat of the Cruiser, Duke (Durham, North Carolina of the U.S.) master of science in global health students Laura Pulscher and Thomas Moore braced themselves during the ride as best they could, relaxing when Inka stopped the vehicle to ask a goat herder for directions.
Pulscher, unfazed by hours of jouncing, said slowly with a matter-of-fact smile, “We’re lost.”
It’s a commonly uttered phrase for visitors and natives alike. A meager 10 percent of Mongolia’s road network is paved. The vast majority of travel in this vast, storied land of Genghis Khan is done on dirt trails and grassy paths.
“If you had asked me before beginning the global health master’s program that I’d be coming to Mongolia, I would have laughed,” Moore would say later at a campsite on the edge of a livestock pasture.
But Mongolia was the opportunity presented to Moore and Pulscher to conduct their field research projects, and they both barely blinked before saying yes.
Early in their three-month stay in Mongolia, Moore and Pulscher spent three weeks traveling north from the capital of Ulaanbaatar toward the Siberian border. With them in a second vehicle were veterinarians Myagmarsuk “Myagaa” Yondon; Igori “Khatnaa” Khatanbaatar and Purevdorj “Zula” Munkhzul from the Mongolian Institute of Veterinary Medicine, who collected blood samples from herders’ horses, goats, and sheep. Moore and Pulscher captured small rodents – Mongolian gerbils, Daurian ground squirrels, Siberian chipmunks, hamsters and field mice – in humane, live animal traps, and injected them with a mild sedative.
Pulscher collected blood and serum samples from the animals’ tails and took small ear biopsies. Moore’s work was far more tedious – combing the oft-matted fur of the rodents, in search of ticks.
Their individual projects differed, but the students’ goals were the same: to detect in rodents’ blood and ticks the presence of dangerous pathogens – Borrelia (Lyme disease), Rickettsia (spotted fever) and anaplasma (blood disease seen mostly in livestock but can cause mild fever in humans) – and share lab results with local health officials and veterinarians who could take prophylactic measures, such as educating herders and their families about ways to protect themselves and their livestock.
In Mongolia, animals have near royalty status. It is a nation of just 3 million people and more than 50 million horses, camels, sheep, goats, and yaks, which are serious players in the economic cycle, and critical for the more than one million herder families who feed off their livestock and make a living selling animals for meat, hides or wool.
“Observing these diseases as they circulate throughout wildlife and livestock has a tremendous impact on the health and welfare of humans here,” Moore said.
Holed up for two days in the dome tent she shared with Sola as an atypical summer downpour drenched the steppe, Pulscher was confident the rain would subside and the rodent trapping continue. Moore entered the tent and announced, “My tent has turned into a swimming pool.”
By afternoon the sun reappeared and the drying-out process commenced. The quest for rodents resumed about a half-hour from camp on a tract of land where a herd of horses grazed nearby. Small, rectangular, aluminum traps were set. When the researchers returned early that evening (the summer sun sets around 10 pm) the sound of scratching was heard coming from within several of them.
“It’s great that we still have a project,” Pulscher jested as she suited up in a full-body Tyvek suit to protect her from any pathogens the rodents might harbor. “I’m glad it stopped raining.”
The veterinarian Khatnaa, with heavily gloved hands, cradled a Mongolian gerbil taken from one of the traps. Pulscher – with Khatnaa’s guidance – injected it with the mild sedative. It was Khatnaa, with his well-spoken English, who had baptized the students to rodent testing in the field. He taught them how to safely handle the animals, determine the correct dose of sedative, clip the tail and ear so as not to cause lasting injury, and collect the blood and tick samples.
After four gerbils were tested and gear repacked inside the Land Cruiser, Moore considered the importance that the team of Mongolian vets played in the success of his and Pulscher’s respective projects.
“They are a tremendous help to us,” Moore said. “They not only teach us different tricks of the trade to catch rodents here, but they act as interpreters when we meet a herder family and provide us with local knowledge of the different species of animals.”
Analyze, Synthesize, Share
The Institute of Veterinary Medicine in Ulaanbaatar is a 1980s-era Soviet-looking building that houses multiple labs with Mongolian scientists exploring various aspects of zoonotic diseases – those that can or might some day jump from animals to humans. This was home base for Moore and Pulscher for the bulk of their three-month Mongolian stay. It was here that the blood and tick samples they collected in the field were analyzed for signs of the dangerous pathogens.
The Duke students extracted DNA from the samples using magnetic beads inside small test tubes. Because DNA has a charge, it is attracted to the beads, which are then separated from the rest of the sample and the DNA extracted.
Performing field and lab work is usually an either/or proposition for students interested in global health. Rarely do master’s students have the opportunity to do both.
“It’s unique that we’re able to collect our own specimens in the environment and bring them back and test them,” Moore said. “This takes our education to the next level.”
“It makes us more well-rounded individuals,” Pulscher added. “A lot of people don’t necessarily have both lab and field skills.”
They worked in the lab with Myagaa, who accompanied them on their fieldwork. His own project involved collecting mosquitoes and testing them for West Nile virus.
Learning about his work, and watching the other Mongolian vets in the field lasso livestock and take blood samples was an unexpected, welcomed educational component, they said.